Affinity Purification of Helicobacter pyloriUrease
نویسندگان
چکیده
منابع مشابه
Affinity purification
Stereomicroscope The new Carl Zeiss stereomicroscope Stemi 2000-CS allows the user to view the sample whilst other observers view the procedure by video. Greenough type stereomicroscopes create their stereo effect by having separate light paths set at different angles, one for each eye. However, when video or photomicrography is being carried out most stereomicroscope systems remove light from ...
متن کاملReceptor affinity purification of a lipid-binding adhesin from Helicobacter pylori.
Our previous work has shown that Helicobacter pylori specifically recognizes gangliotetraosylceramide, gangliotriaosylceramide, and phosphatidylethanolamine in vitro. This binding specificity is shared by exoenzyme S from Pseudomonas aeruginosa, and monoclonal antibodies against this adhesin prevent the attachment of H. pylori to its lipid receptors. We now report the use of a novel, versatile ...
متن کاملAffinity purification of Helicobacter pylori urease. Relevance to gastric mucin adherence by urease protein.
A simple, reproducible and high yield method of Helicobacter pylori urease enzyme purification was developed using a heparinoid (Cellufine sulfate) affinity gel. The purification method involved two sequential steps using the same gel that takes advantage of the differential affinity of urease to the heparinoid at two levels of hydrogen ion concentration. SDS-polyacrylamide gel electrophoresis ...
متن کاملPurification by Affinity Chromatography
The glycine receptor of rat spinal cord was solubilized with the nonionic detergent Triton X-100 and subsequently purified by affinity chromatography on aminostrychnine-agarose and wheat germ agglutininSepharose. An overall purification of 1950-fold was achieved. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and mercaptoethano1 revealed three glycine receptor-asso...
متن کاملAffinity purification of protein complexes.
INTRODUCTION Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest....
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.29.18130